abstract：:Inhibitory antibodies directed against blood coagulation factor VIII (FVIII) impair FVIII replacement therapy, constituting a serious complication in haemophilic and autoimmune patients. Identifying B-cell FVIII epitopes and mapping them on the molecule remain important challenges. Using a combination of different alg...

journal_title：Molecular immunology

authors： Di Giambattista M,Branckaert T,Hougardy V,Kemball-Cook G,Laub R

更新日期：2007-03-01 00:00:00

abstract：:Several mutations in the CFH gene have been described in non-Shiga-toxin-associated haemolytic uraemic syndrome (non-Stx-HUS), a rare syndrome characterized by haemolytic anaemia, thrombocytopenia and acute renal failure. Mutations in genes encoding other complement regulatory proteins, membrane cofactor protein (CD46...

journal_title：Molecular immunology

authors： Monteferrante G,Brioschi S,Caprioli J,Pianetti G,Bettinaglio P,Bresin E,Remuzzi G,Noris M

更新日期：2007-03-01 00:00:00

abstract：:The chemokine receptor CCR2 binds four pro-inflammatory monocyte chemoattractant proteins, designated MCP1/CCL2, MCP2/CCL8, MCP3/CCL7 and MCP4/CCL13. This study demonstrates the important biology of this receptor during the response to the chemokine milieu. Competitive chemotaxis and calcium flux assays were performed...

journal_title：Molecular immunology

authors： O'Boyle G,Brain JG,Kirby JA,Ali S

更新日期：2007-03-01 00:00:00

abstract：:Hepcidins are antimicrobial peptides that play important roles in resisting pathogenic infection. Through hybridization of a phage library, the cDNA sequences of three hepcidin-like antimicrobial peptides (named TH1-5, TH2-2, and TH2-3) in tilapia, Oreochromis mossambicus, were determined. The complete hepcidin cDNA s...

journal_title：Molecular immunology

authors： Huang PH,Chen JY,Kuo CM

更新日期：2007-03-01 00:00:00

abstract：:Prohibitin (PHB1) is a multifunction protein that is released in lipid droplets from adipocytes and possibly other cells and is detectable in the circulation. We used crosslinking, immunoprecipitation and proteomic analysis to investigate binding partners for circulating PHB1. Crosslinking of PHB1 to serum resulted in...

journal_title：Molecular immunology

authors： Mishra S,Moulik S,Murphy LJ

更新日期：2007-03-01 00:00:00

abstract：:MTX is an effective therapy for autoimmune-inflammatory diseases. The mechanisms that mediate these actions are not completely clear. It is accepted that many of these effects are mediated through the release of adenosine with the activation of the adenosine receptor A2. MTX is used as a steroid sparing agent. An impr...

journal_title：Molecular immunology

authors： Goecke IA,Alvarez C,Henríquez J,Salas K,Molina ML,Ferreira A,Gatica H

更新日期：2007-03-01 00:00:00

abstract：:The T cell stimulatory activity of peptides is known to be associated with the cell surface stability and lifetime of the peptide-MHC (pepMHC) complex. In this report, soluble high-affinity T cell receptors (TCRs) that are specific for pepMHC complexes recognized by the mouse CD8+ clone 2C were used to monitor the cel...

journal_title：Molecular immunology

authors： Brophy SE,Jones LL,Holler PD,Kranz DM

更新日期：2007-03-01 00:00:00

abstract：:The anti-cytokeratin (CK) 8 monoclonal antibody (mab) TS1 has been shown to efficiently bind to CK8 expressed in carcinomas in vivo. The anti-idiotypic antibody of TS1, alphaTS1, can be used to regulate the tumor:non-tumor ratio of TS1 by clearing non-tumor binding TS1 from the circulation. If the interaction of TS1 t...

journal_title：Molecular immunology

authors： Holm P,Jafari R,Sundström BE

更新日期：2007-02-01 00:00:00

abstract：:Cardamonin, a chalcone isolated from the fruits of a local plant Alpinia rafflesiana, has demonstrated anti-inflammatory activity in cellular models of inflammation. In this report, we evaluated the ability of cardamonin to suppress both NO and PGE2 synthesis, iNOS and COX-2 expression and enzymatic activity, and key ...

journal_title：Molecular immunology

authors： Israf DA,Khaizurin TA,Syahida A,Lajis NH,Khozirah S

更新日期：2007-02-01 00:00:00

abstract：:Many infections are associated with diabetes, as the ability of the body to fight pathogens is impaired. Recently, altered levels of defensins and polymorphism in Toll-like receptors (TLRs), two crucial components of the innate immune system, have been associated with diabetes. To study the functionality of the innate...

journal_title：Molecular immunology

authors： Froy O,Hananel A,Chapnik N,Madar Z

更新日期：2007-02-01 00:00:00

abstract：:C1q, the recognition molecule of the classical pathway of complement, binds to endothelial cells, leading to cell activation. Mannose-binding lectin (MBL), a recognition molecule of the lectin pathway, is structurally and functionally related to C1q. Therefore, we investigated the interaction of MBL with human umbilic...

journal_title：Molecular immunology

authors： Oroszlán M,Daha MR,Cervenak L,Prohászka Z,Füst G,Roos A

更新日期：2007-02-01 00:00:00

abstract：:This paper reports the cloning and sequencing of two pro-inflammatory cytokines, interleukin (IL)-1beta and IL-8, in haddock (Melanogrammus aeglefinus) by homology cloning. The complete transcript of the haddock IL-1beta was sequenced and contained 1043 bp, including a 762 bp open reading frame. The 3' end of the gene...

journal_title：Molecular immunology

authors： Corripio-Miyar Y,Bird S,Tsamopoulos K,Secombes CJ

更新日期：2007-02-01 00:00:00

abstract：:A high affinity C5 convertase is generated when a C3 convertase deposits additional C3b molecules on and around itself thereby switching the substrate specificity of C3 convertase from C3 to C5. In the present study the role of the additional C3b molecules in influencing the regulation of classical pathway C5 converta...

journal_title：Molecular immunology

authors： Rawal N,Pangburn MK

更新日期：2007-02-01 00:00:00

abstract：:Attempts to develop peptide vaccines, based on a limited number of peptides face two problems: HLA polymorphism and the high mutation rate of viral epitopes. We have developed a new genomic method that ensures maximal coverage and thus maximal applicability of the peptide vaccine. The same method also promises a large...

journal_title：Molecular immunology

authors： Vider-Shalit T,Raffaeli S,Louzoun Y

更新日期：2007-02-01 00:00:00

abstract：:Fibrates, which function by binding and activating peroxisome proliferator-activated receptor alpha (PPARalpha), have been used successfully to treat hyperlipidemia and atherosclerosis. Increasing evidence suggests that in addition to their lipid lowering activities these medications also function as immunosuppressive...

journal_title：Molecular immunology

authors： Selim E,Frkanec JT,Cunard R

更新日期：2007-02-01 00:00:00

abstract：:Chlorophyllin (CHL) was earlier shown to reduce the level of intracellular ROS and apoptosis induced by ionizing radiation and 2,2'-azobis(2-propionimidinedihydrochloride) (AAPH). In the present studies, the effect of CHL on radiation-induced immunosuppression and modulation of immune responses in mice was examined. C...

journal_title：Molecular immunology

authors： Sharma D,Kumar SS,Sainis KB

更新日期：2007-01-01 00:00:00

abstract：:Selection of specific antibodies from large repertoires is of importance in generating antibodies to specific structural determinants and in studying structure-function relationships. Alkaline phosphatase (AP) has several isozymes with various degrees of homology and a range of common synthetic substrates. We have pre...

journal_title：Molecular immunology

authors： Jain V,Saini D,Goswami P,Sinha S

更新日期：2007-01-01 00:00:00

abstract：:The carbohydrate binding properties of a novel member of the subfamily of galactose-specific jacalin-related lectin isolated from the bark of black mulberry (Morus nigra) (Morniga G) was studied in detail by enzyme-linked lectinosorbent and inhibition assays using panels of monomeric saccharides, mammalian polyvalent ...

journal_title：Molecular immunology

authors： Singh T,Wu JH,Peumans WJ,Rougé P,Van Damme EJ,Wu AM

更新日期：2007-01-01 00:00:00

abstract：:The integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the NADPH oxidase complex, a multicomponent enzyme system that initiates a cascade of reactive oxygen species that play a critical role in innate immunity and vascular physiology. Epitope-mapped, monoclonal antibodies (mAb) that recognize...

journal_title：Molecular immunology

authors： Taylor RM,Maaty WS,Lord CI,Hamilton T,Burritt JB,Bothner B,Jesaitis AJ

更新日期：2007-01-01 00:00:00

abstract：:Pulmonary exposure to innocuous aeroallergens is a common event leading to inhalation tolerance. Distinct subsets of pulmonary dendritic cells (DC) and regulatory T cells (T(Reg)) play critical roles in mediating and maintaining such tolerance. In asthmatics, the same aeroallergens drive a maladaptive, Th2-biased immu...

journal_title：Molecular immunology

authors： Köhl J,Wills-Karp M

更新日期：2007-01-01 00:00:00

abstract：:In humans, the CD4 molecule is expressed on a subset of T-cells and at various levels on myeloid and lymphoid cells. The mechanisms regulating human CD4 gene expression are yet poorly understood. We speculated that the CD4 silencer, which operates in CD8+ T-cells to repress CD4 expression, could be responsible for CD4...

journal_title：Molecular immunology

authors： Mouly E,Dorival C,Pflumio F,Baillou C,Coulombel L,Levy Y,Lemoine FM,Klatzmann D,Marodon G

更新日期：2007-01-01 00:00:00

abstract：:The ectodomain of matrix protein 2 (M2e) has remained remarkably conserved amongst human influenza A viruses and is a target for Abs with protective activity. For these reasons, M2e is being investigated for its potential as a broadly protective influenza A virus vaccine. Here, we report on the fine specificity and se...

journal_title：Molecular immunology

authors： Zhang M,Zharikova D,Mozdzanowska K,Otvos L,Gerhard W

更新日期：2006-07-01 00:00:00

abstract：:Cleavage by the proteasome is responsible for generating the C terminus of T-cell epitopes. Modeling the process of proteasome cleavage as part of a multi-step algorithm for T-cell epitope prediction will reduce the number of non-binders and increase the overall accuracy of the predictive algorithm. Quantitative matri...

journal_title：Molecular immunology

authors： Doytchinova IA,Flower DR

更新日期：2006-05-01 00:00:00

abstract：:The DNA vaccine based on the glycoprotein gene of Infectious hematopoietic necrosis virus induces a non-specific anti-viral immune response and long-term specific immunity against IHNV. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injec...

journal_title：Molecular immunology

authors： Purcell MK,Nichols KM,Winton JR,Kurath G,Thorgaard GH,Wheeler P,Hansen JD,Herwig RP,Park LK

更新日期：2006-05-01 00:00:00

abstract：:Antibacterial responses have been studied in Atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroA(-)) strain of Aeromonas salmonicida known to elicit protective immunity. Three tissues were studied for transcriptional changes, the liver, head kidney and the gill. RNA was coll...

journal_title：Molecular immunology

authors： Martin SA,Blaney SC,Houlihan DF,Secombes CJ

更新日期：2006-04-01 00:00:00

abstract：:REV1 plays a key role in vertebrate translesion synthesis. Although its deoxycytidyl transferase activity is dispensable for tolerance of DNA damage caused by a number of mutagens, its extreme C terminus, which interacts with other translesion polymerases and PCNA, is essential. By examining immunoglobulin diversifica...

journal_title：Molecular immunology

authors： Ross AL,Sale JE

更新日期：2006-04-01 00:00:00

abstract：:Withanolide, coagulin-H (1), was evaluated for its effect on various cellular functions related to immune response including lymphocyte proliferation, and expression of interleukin-2 (IL-2) cytokine, and results were compared with prednisolone (2), a commonly used immune modulating drug. Coagulin-H (1) was found to ha...

journal_title：Molecular immunology

authors： Mesaik MA,Zaheer-Ul-Haq,Murad S,Ismail Z,Abdullah NR,Gill HK,Atta-Ur-Rahman,Yousaf M,Siddiqui RA,Ahmad A,Choudhary MI

更新日期：2006-04-01 00:00:00

abstract：:Somatic hypermutation (SHM) in immunoglobulin gene (Ig) variable (V) regions is critical for the maturation of the antibody response. It is dependent on the expression of activation-induced cytidine deaminase (AID) and translesion DNA polymerases in germinal center B cells as well as Ig V transcription, as regulated b...

journal_title：Molecular immunology

authors： Komori A,Xu Z,Wu X,Zan H,Casali P

更新日期：2006-04-01 00:00:00

abstract：:CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. In this study, we describe the isolation of the full-length cDNA encoding for CD9 molecule (daCD9) of red stingray, Dasyatis akajei. This 1252 bp cDNA was isolated from leukocyte cDNA library and contains 68...

journal_title：Molecular immunology

authors： Zhu J,Yan K,Lu L,Peng C,Zhou C,Chen S,Xie X,Dong M,Xu A

更新日期：2006-04-01 00:00:00

abstract：:Protein kinase C-associated kinase (PKK; DIK/RIP4) is an ankyrin-repeat containing serine/threonine receptor-interacting protein (RIP)-family kinase that can activate NFkappaB, and is required for keratinocyte development. In earlier studies, the expression of a catalytically inactive mutant of PKK in the B cell linea...

journal_title：Molecular immunology

authors： Moran ST,Cariappa A,Liu H,Boboila C,Shi HN,Holland PM,Peschon JJ,Pillai S

更新日期：2006-04-01 00:00:00

abstract：:The innate immune response is critical to enteric disease resistance and the induction of mucosal adaptive immunity. In mucosae of the small intestine, Peyer's patches play a central role in immune surveillance and sampling of bacteria by specialized M cells. The innate immune response to Salmonella enterica serovar C...

journal_title：Molecular immunology

authors： Hyland KA,Kohrt L,Vulchanova L,Murtaugh MP

更新日期：2006-04-01 00:00:00

abstract：:With the exception of a few strains such as BuB/BnJ, complement activity in most inbred strains of mice is remarkably similar. The BuB/BnJ strain reportedly has high levels of complement activity and elevated serum levels of C3 and other complement proteins. However, we observed that BuB/BnJ mice have serum C3 levels ...

journal_title：Molecular immunology

authors： Osmers I,Szalai AJ,Tenner AJ,Barnum SR

更新日期：2006-04-01 00:00:00

abstract：:Epithelial surfaces constitute natural immunobarriers against environmental threats. These barriers are brimming with fluids that bind, transport, cleave or degrade bacterial cells and their endotoxic by-products. Saliva and the airway surface-lining fluid (ASL) comprise the important fluid constituents. Short palate,...

journal_title：Molecular immunology

authors： Zhou HD,Li GY,Yang YX,Li XL,Sheng SR,Zhang WL,Zhao J

更新日期：2006-04-01 00:00:00

abstract：:Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. The ...

journal_title：Molecular immunology

authors： Hauton C,Brockton V,Smith VJ

更新日期：2006-03-01 00:00:00

abstract：:A study aiming at cloning and characterizing natural antibodies to human immunodeficiency virus type 1 (HIV-1) targets is described. In particular, we report the molecular cloning of a Fab molecule binding the HIV-1/Tat protein from a seronegative patient. The Fab was characterized for its binding specificity and inve...

journal_title：Molecular immunology

authors： Mancini N,Perotti M,Carletti S,Canducci F,Sampaolo M,Clementi M,Burioni R

更新日期：2006-03-01 00:00:00

abstract：:A common feature of human IgG1 antibodies used for cancer treatment is that their anti-tumour efficacy requires high serum trough levels and continued therapy for several months. Treatment cycles, thereby, consume several grams of IgG1 translating into significant drug needs and costs. The basis for the low in vivo ef...

journal_title：Molecular immunology

authors： Preithner S,Elm S,Lippold S,Locher M,Wolf A,da Silva AJ,Baeuerle PA,Prang NS

更新日期：2006-03-01 00:00:00

abstract：:Rearrangements in reading frame 2 promote the expression of a truncated heavy chain, the Dmu protein. Dmu can assemble into a pre-B cell receptor like complex that appears to induce a subset of signals elicited by full length mu, but cannot promote the pro-B to pre-B cell transition of Rag-/- B cells. In order to dete...

journal_title：Molecular immunology

authors： Wikström I,Bergqvist I,Holmberg D,Forssell J

更新日期：2006-03-01 00:00:00

abstract：:Human neutrophil peptides (HNPs) are cysteine-rich antimicrobial peptides stored in neutrophils. The similar structure of HNPs -1, -2, and -3 renders them impossible to study individually in biological samples. For the first time, we describe a method of individually identifying the HNPs -1-3 from exudative neutrophil...

journal_title：Molecular immunology

authors： Thompson L,Turko I,Murad F

更新日期：2006-03-01 00:00:00

abstract：:The expression levels of three commonly used housekeeping genes, EF1-alpha, RPS20 and Beta-Actin, were examined in seven different tissues and leucocytes from non-stimulated Atlantic salmon (Salmo salar L.). The tissues analysed by quantitative real-time PCR were gill, liver, intestine, muscle, spleen, head kidney leu...

journal_title：Molecular immunology

authors： Ingerslev HC,Pettersen EF,Jakobsen RA,Petersen CB,Wergeland HI

更新日期：2006-03-01 00:00:00

abstract：:Dendritic cells (DCs) are recruited to inflammatory sites where they phagocytose and process antigens for subsequent presentation to the T lymphocytes in the lymphoid tissue. Several leukocyte chemoattractants and their specific receptors have been shown to induce the migration of DC. The complement protein C1q has mu...

journal_title：Molecular immunology

authors： Vegh Z,Kew RR,Gruber BL,Ghebrehiwet B

更新日期：2006-03-01 00:00:00